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polyclonal rabbit anti human leptin y20  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology polyclonal rabbit anti human leptin y20
    Figure 1 Inhibition of NFκB suppressed E2 effect on <t>leptin</t> expression BeWo cells (A) and (C) and term placental explants (B) and (D) were pretreated as indicated with sulfasalazine during 30 min, afterwards E2 was added. After 48 h (A) or 24 h (B), total RNA was extracted and Leptin mRNA expression was quantified by qRT-PCR. Cyclophilin was used as internal standard. Cell extracts (C) and placental explants extracts (D) were prepared as indicated in ‘Materials and methods’ section and proteins were separated on 12% SDS-PAGE gel. Leptin expression was determined by Western blot. Loading control was performed by immunoblotting the same membranes with anti-GAPDH. Standard protein markers were used to estimate the molecular weights. Molecular weight (kDa) is indicated at the right of each blot. Bands densitometry is shown in lower panels. Results are expressed as mean ± s.d. for ten independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control and ##P < 0.01, ###P < 0.001 vs E2 treatment. Statistical analyses were performed by ANOVA followed by Bonferroni post hoc test.
    Polyclonal Rabbit Anti Human Leptin Y20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+human+leptin+y20/10__1530_slash_rep___20___0142-90-31-37?v=Santa+Cruz+Biotechnology
    Average 95 stars, based on 340 article reviews
    polyclonal rabbit anti human leptin y20 - by Bioz Stars, 2026-07
    95/100 stars

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    1) Product Images from "Crosstalk between estradiol and NFκB signaling pathways on placental leptin expression"

    Article Title: Crosstalk between estradiol and NFκB signaling pathways on placental leptin expression

    Journal: Reproduction

    doi: 10.1530/rep-20-0142

    Figure 1 Inhibition of NFκB suppressed E2 effect on leptin expression BeWo cells (A) and (C) and term placental explants (B) and (D) were pretreated as indicated with sulfasalazine during 30 min, afterwards E2 was added. After 48 h (A) or 24 h (B), total RNA was extracted and Leptin mRNA expression was quantified by qRT-PCR. Cyclophilin was used as internal standard. Cell extracts (C) and placental explants extracts (D) were prepared as indicated in ‘Materials and methods’ section and proteins were separated on 12% SDS-PAGE gel. Leptin expression was determined by Western blot. Loading control was performed by immunoblotting the same membranes with anti-GAPDH. Standard protein markers were used to estimate the molecular weights. Molecular weight (kDa) is indicated at the right of each blot. Bands densitometry is shown in lower panels. Results are expressed as mean ± s.d. for ten independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control and ##P < 0.01, ###P < 0.001 vs E2 treatment. Statistical analyses were performed by ANOVA followed by Bonferroni post hoc test.
    Figure Legend Snippet: Figure 1 Inhibition of NFκB suppressed E2 effect on leptin expression BeWo cells (A) and (C) and term placental explants (B) and (D) were pretreated as indicated with sulfasalazine during 30 min, afterwards E2 was added. After 48 h (A) or 24 h (B), total RNA was extracted and Leptin mRNA expression was quantified by qRT-PCR. Cyclophilin was used as internal standard. Cell extracts (C) and placental explants extracts (D) were prepared as indicated in ‘Materials and methods’ section and proteins were separated on 12% SDS-PAGE gel. Leptin expression was determined by Western blot. Loading control was performed by immunoblotting the same membranes with anti-GAPDH. Standard protein markers were used to estimate the molecular weights. Molecular weight (kDa) is indicated at the right of each blot. Bands densitometry is shown in lower panels. Results are expressed as mean ± s.d. for ten independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control and ##P < 0.01, ###P < 0.001 vs E2 treatment. Statistical analyses were performed by ANOVA followed by Bonferroni post hoc test.

    Techniques Used: Inhibition, Expressing, Quantitative RT-PCR, SDS Page, Western Blot, Control, Molecular Weight

    Figure 2 Effect of p65 overexpression on E2 induced placental leptin expression. (A) BeWo cells were transiently transfected with pL1951 plasmid construction containing the promoter region of leptin gene from −1951 to +42 bp and different amounts of p65 expression plasmid. After transfection, BeWo cells were incubated for 48 h in DMEM-F12 media supplemented with 1% FBS and treated with E2 as indicated. (B) BeWo cells were transiently transfected with pL1951 plasmid construction, different amounts of plasmid expressing human
    Figure Legend Snippet: Figure 2 Effect of p65 overexpression on E2 induced placental leptin expression. (A) BeWo cells were transiently transfected with pL1951 plasmid construction containing the promoter region of leptin gene from −1951 to +42 bp and different amounts of p65 expression plasmid. After transfection, BeWo cells were incubated for 48 h in DMEM-F12 media supplemented with 1% FBS and treated with E2 as indicated. (B) BeWo cells were transiently transfected with pL1951 plasmid construction, different amounts of plasmid expressing human

    Techniques Used: Over Expression, Expressing, Transfection, Plasmid Preparation, Incubation



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    polyclonal rabbit anti human leptin y20  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology polyclonal rabbit anti human leptin y20
    Figure 1 Inhibition of NFκB suppressed E2 effect on <t>leptin</t> expression BeWo cells (A) and (C) and term placental explants (B) and (D) were pretreated as indicated with sulfasalazine during 30 min, afterwards E2 was added. After 48 h (A) or 24 h (B), total RNA was extracted and Leptin mRNA expression was quantified by qRT-PCR. Cyclophilin was used as internal standard. Cell extracts (C) and placental explants extracts (D) were prepared as indicated in ‘Materials and methods’ section and proteins were separated on 12% SDS-PAGE gel. Leptin expression was determined by Western blot. Loading control was performed by immunoblotting the same membranes with anti-GAPDH. Standard protein markers were used to estimate the molecular weights. Molecular weight (kDa) is indicated at the right of each blot. Bands densitometry is shown in lower panels. Results are expressed as mean ± s.d. for ten independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control and ##P < 0.01, ###P < 0.001 vs E2 treatment. Statistical analyses were performed by ANOVA followed by Bonferroni post hoc test.
    Polyclonal Rabbit Anti Human Leptin Y20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+human+leptin+y20/10__1530_slash_rep___20___0142-90-31-37?v=Santa+Cruz+Biotechnology
    Average 95 stars, based on 1 article reviews
    polyclonal rabbit anti human leptin y20 - by Bioz Stars, 2026-07
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    polyclonal rabbit anti-human leptin y20 (1 : 1000) antibodies  (Millipore)
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    Millipore polyclonal rabbit anti-human leptin y20 (1 : 1000) antibodies
    Figure 1 Inhibition of NFκB suppressed E2 effect on <t>leptin</t> expression BeWo cells (A) and (C) and term placental explants (B) and (D) were pretreated as indicated with sulfasalazine during 30 min, afterwards E2 was added. After 48 h (A) or 24 h (B), total RNA was extracted and Leptin mRNA expression was quantified by qRT-PCR. Cyclophilin was used as internal standard. Cell extracts (C) and placental explants extracts (D) were prepared as indicated in ‘Materials and methods’ section and proteins were separated on 12% SDS-PAGE gel. Leptin expression was determined by Western blot. Loading control was performed by immunoblotting the same membranes with anti-GAPDH. Standard protein markers were used to estimate the molecular weights. Molecular weight (kDa) is indicated at the right of each blot. Bands densitometry is shown in lower panels. Results are expressed as mean ± s.d. for ten independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control and ##P < 0.01, ###P < 0.001 vs E2 treatment. Statistical analyses were performed by ANOVA followed by Bonferroni post hoc test.
    Polyclonal Rabbit Anti Human Leptin Y20 (1 : 1000) Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+human+leptin+y20/pm23067361-57-17-28?v=Millipore
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit anti-human leptin y20 (1 : 1000) antibodies - by Bioz Stars, 2026-07
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    Figure 1 Inhibition of NFκB suppressed E2 effect on leptin expression BeWo cells (A) and (C) and term placental explants (B) and (D) were pretreated as indicated with sulfasalazine during 30 min, afterwards E2 was added. After 48 h (A) or 24 h (B), total RNA was extracted and Leptin mRNA expression was quantified by qRT-PCR. Cyclophilin was used as internal standard. Cell extracts (C) and placental explants extracts (D) were prepared as indicated in ‘Materials and methods’ section and proteins were separated on 12% SDS-PAGE gel. Leptin expression was determined by Western blot. Loading control was performed by immunoblotting the same membranes with anti-GAPDH. Standard protein markers were used to estimate the molecular weights. Molecular weight (kDa) is indicated at the right of each blot. Bands densitometry is shown in lower panels. Results are expressed as mean ± s.d. for ten independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control and ##P < 0.01, ###P < 0.001 vs E2 treatment. Statistical analyses were performed by ANOVA followed by Bonferroni post hoc test.

    Journal: Reproduction

    Article Title: Crosstalk between estradiol and NFκB signaling pathways on placental leptin expression

    doi: 10.1530/rep-20-0142

    Figure Lengend Snippet: Figure 1 Inhibition of NFκB suppressed E2 effect on leptin expression BeWo cells (A) and (C) and term placental explants (B) and (D) were pretreated as indicated with sulfasalazine during 30 min, afterwards E2 was added. After 48 h (A) or 24 h (B), total RNA was extracted and Leptin mRNA expression was quantified by qRT-PCR. Cyclophilin was used as internal standard. Cell extracts (C) and placental explants extracts (D) were prepared as indicated in ‘Materials and methods’ section and proteins were separated on 12% SDS-PAGE gel. Leptin expression was determined by Western blot. Loading control was performed by immunoblotting the same membranes with anti-GAPDH. Standard protein markers were used to estimate the molecular weights. Molecular weight (kDa) is indicated at the right of each blot. Bands densitometry is shown in lower panels. Results are expressed as mean ± s.d. for ten independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control and ##P < 0.01, ###P < 0.001 vs E2 treatment. Statistical analyses were performed by ANOVA followed by Bonferroni post hoc test.

    Article Snippet: Membranes were equilibrated in 1× PBS and non-specific binding sites were blocked with 5% non-fat milk in PBS at room temperature for 1 h. Then they were immunoblotted with the specific polyclonal rabbit anti-human leptin Y20 (1:1000, Santa Cruz Biotechnology, Inc.), monoclonal mouse anti-p65 (1:1000, Santa Cruz Biotechnology, Inc.), or monoclonal mouse anti-pIκBα (1:1000 Santa Cruz Biotechnology, Inc.).

    Techniques: Inhibition, Expressing, Quantitative RT-PCR, SDS Page, Western Blot, Control, Molecular Weight

    Figure 2 Effect of p65 overexpression on E2 induced placental leptin expression. (A) BeWo cells were transiently transfected with pL1951 plasmid construction containing the promoter region of leptin gene from −1951 to +42 bp and different amounts of p65 expression plasmid. After transfection, BeWo cells were incubated for 48 h in DMEM-F12 media supplemented with 1% FBS and treated with E2 as indicated. (B) BeWo cells were transiently transfected with pL1951 plasmid construction, different amounts of plasmid expressing human

    Journal: Reproduction

    Article Title: Crosstalk between estradiol and NFκB signaling pathways on placental leptin expression

    doi: 10.1530/rep-20-0142

    Figure Lengend Snippet: Figure 2 Effect of p65 overexpression on E2 induced placental leptin expression. (A) BeWo cells were transiently transfected with pL1951 plasmid construction containing the promoter region of leptin gene from −1951 to +42 bp and different amounts of p65 expression plasmid. After transfection, BeWo cells were incubated for 48 h in DMEM-F12 media supplemented with 1% FBS and treated with E2 as indicated. (B) BeWo cells were transiently transfected with pL1951 plasmid construction, different amounts of plasmid expressing human

    Article Snippet: Membranes were equilibrated in 1× PBS and non-specific binding sites were blocked with 5% non-fat milk in PBS at room temperature for 1 h. Then they were immunoblotted with the specific polyclonal rabbit anti-human leptin Y20 (1:1000, Santa Cruz Biotechnology, Inc.), monoclonal mouse anti-p65 (1:1000, Santa Cruz Biotechnology, Inc.), or monoclonal mouse anti-pIκBα (1:1000 Santa Cruz Biotechnology, Inc.).

    Techniques: Over Expression, Expressing, Transfection, Plasmid Preparation, Incubation